ABSTRACT
Salinity is a pivotal abiotic stress factor with far-reaching consequences on global crop growth, yield, and quality and which includes strawberries. R2R3-MYB transcription factors encompass a range of roles in plant development and responses to abiotic stress. In this study, we identified that strawberry transcription factor FaMYB63 exhibited a significant upregulation in its expression under salt stress conditions. An analysis using yeast assay demonstrated that FaMYB63 exhibited the ability to activate transcriptional activity. Compared with those in the wild-type (WT) plants, the seed germination rate, root length, contents of chlorophyll and proline, and antioxidant activities (SOD, CAT, and POD) were significantly higher in FaMYB63-overexpressing Arabidopsis plants exposed to salt stress. Conversely, the levels of malondialdehyde (MDA) were considerably lower. Additionally, the FaMYB63-overexpressed Arabidopsis plants displayed a substantially improved capacity to scavenge active oxygen. Furthermore, the activation of stress-related genes by FaMYB63 bolstered the tolerance of transgenic Arabidopsis to salt stress. It was also established that FaMYB63 binds directly to the promoter of the salt overly sensitive gene SOS1, thereby activating its expression. These findings identified FaMYB63 as a possible and important regulator of salt stress tolerance in strawberries.
Subject(s)
Arabidopsis Proteins , Arabidopsis , Gene Expression Regulation, Plant , Plants, Genetically Modified , Salt Tolerance , Sodium-Hydrogen Exchangers , Transcription Factors , Arabidopsis/genetics , Arabidopsis/physiology , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Plants, Genetically Modified/genetics , Plants, Genetically Modified/physiology , Salt Tolerance/genetics , Sodium-Hydrogen Exchangers/genetics , Transcription Factors/genetics , Transcription Factors/metabolism , Fragaria/geneticsABSTRACT
It has been proved that the nitrogen can be removed from the sediment in a sediment microbial fuel cell system (SMFCs), but the competition between nitrate and oxygen for electrons would be a key factor that would affect the removal efficiency, and its mechanism is not clear. Based on organic sediment fuel, an SMFC was constructed, and the influence of dissolved oxygen (DO) on nitrogen transformation and cathodic microbial communities was investigated. The results showed that the best total nitrogen removal efficiency of 60.55% was achieved at DO level of 3 mg/L. High DO concentration affected the removal efficiency through the electrons' competition with nitrate, while low DO concentration suppressed the nitrification. Comamonas, Diaphorobacter and Brevundimonas were the three dominant genera responsible for denitrification at DO concentration of 3 mg/L in this study. The establishment of SMFCs for nitrogen removal by regulating DO level would offer a promising method for sediment treatment.
Subject(s)
Bioelectric Energy Sources , Denitrification , Electrons , Nitrification , Nitrogen , OxygenABSTRACT
A piezoelectric bimorph beam, as an upgraded cantilever beam structure, can be used to detect gas content and build a micro-actuator, among other functions. Thus, this beam is widely applied to microelectromechanical systems (MEMS), transformers, and precision machinery. For example, when photoacoustic spectroscopy is performed to detect oil-soluble gas in transformers, a micro-cantilever beam can be used to detect gas content. The dynamic characteristics of piezoelectric bimorph beams, such as resonant frequency, are important indexes in the applications of these beams. The equivalent circuit model for a piezoelectric bimorph beam is examined in this study and an admittance test is performed on the beam to accurately, quickly, and economically measure and analyze its dynamic characteristics. Then, the least squares method is applied to obtain the characteristic curves of the admittance circle, amplitude frequency, and phase frequency; identify the dynamic characteristics of the piezoelectric bimorph beam (e.g., resonant frequency); and determine the parameters of the equivalent circuit. The resonant frequency of the piezoelectric bimorph beam is 207.67 Hz based on the result of the admittance circle test, which is basically consistent with the results of microscope image method (i.e., 207.85 Hz) and the theoretical calculation (i.e., 222.03 Hz). This finding proves the validity of the proposed test method. This method cannot only improve the detection speed of piezoelectric bimorph beams, but can also provide a fast detection strategy for testing the characteristics of such beams during photoacoustic spectroscopy.
